Coezyme A requirement of malaria parasites: effects of coenzyme A precursors on extracellular development in vitro of Plasmodium lophurae.
نویسندگان
چکیده
The extracellular development in vitro of the avian malaria Plasmodium lophurae is favored by addition to the medium of coenzyme A at 0.05 mM. Coenzyme A can be replaced by dephospho-coenzyme A and to some extent by phosphopantetheine, but not by phosphopantothenoylcysteine or by phosphopantothenic acid. The activity of the two former precursors results from their conversion to coenzyme A by enzymes in the erythrocyte extract of the culture medium in the presence of ATP, also an essential ingredient of the medium. Hence, P. lophurae in its erythrocytic stage has an absolute requirement for an exogenous source of coenzyme A.
منابع مشابه
Coenzyme A requirement of malaria parasites: enzymes of coenzyme A biosynthesis in normal duck erythrocytes and erythrocytes infected with Plasmodium lophurae.
Normal duck erythrocytes and erythrocytes infected with Plasmodium lopharae have all of the enzymes for coenzyme A biosynthesis, whereas parasites freed from their host cells have non. Since erythrocytefree cultivation of P. lophurae requires an exogenous source of coenzyme A, this parasite must obtain its coenzyme A entirely from the host cell during infection.
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عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 72 5 شماره
صفحات -
تاریخ انتشار 1975